Feature
Whole genome sequencing (WGS) evaluates the full DNA content of an organism — be it human, plant, animal, or microbe. PacBio HiFi sequencing has exceptionally accurate long reads and unbiased coverage required to provide a truly comprehensive view. HiFi sequencing delivers whole genome sequence information with complete and accurate resolution through unbiased, single-molecule reads of up to 25 kb, a median read accuracy of >99.9% (Q30), and the ability to sequence through repeats and GC-rich regions: Complete, contiguous, correct, and phased genome assemblies。HiFi read length and accuracy resolve even the most difficult repeats — segmental duplications and centromeres — to produce genomes with longer contigs, fewer fragmented genes, and the correct sequence of all regions. Highest precision and recall for variant calling。Only HiFi sequencing enables the highest precision and recall for calling variants of all types: single-nucleotide variants (SNVs), small insertions and deletions (indels), and structural variants (SVs).
Data Delivery
Platform：PacBio Revio
Library：SMRT Cell Library，HiFi
Seq mode：Single Molecule, Real-Time (SMRT) , HiFi mode
Data Size：90G data
Data Output: Reads.bam